Potential of engineered Pseudomonas putida for Bioremediation of Cr (VI) tannery polluted environment
| Author(s) | : | G.Renuga, M. Janeefa |
| Institution | : | Principal & Research co-ordinator, Dept of Biochemistry |
| Published In | : | Vol. 5, Issue 1 — January 2018 |
| Page No. | : | 324-329 |
| Domain | : | Engineering |
| Type | : | Research Paper |
| ISSN (Online) | : | 2348-4470 |
| ISSN (Print) | : | 2348-6406 |
Bioremediation of composite tannery wastewater appears to be incompatible for direct biological treatment.The present work aimed contaminated area can be treated with microbes that are genetically engineered for degradationspecific toxic compounds. Tannery wastewater is heavily polluted containing appreciable biodegradable matters as wellas inorganic substances like chromium, sulphide, chloride etc. Wastewater emanated from various sub-processes oftannery operation showed variable characteristics and also differential treatability.Pseudomonas putida isolated fromtannery sludge, were examined for their tolerance to hexavalent chromium Cr (VI) and their ability to reduce Cr (VI) toCr(III), a detoxification process in cell suspension and cell extracts.. Pseudomonas putida could reduce Cr (VI) up to75% from the total content of different level of dilutions could confirm in terms of their Cr (VI) reducing ability andresistance to Cr (VI), and the Cr (VI) reduction was mainly associated with the soluble protein fraction of the cell..P.putida used as a source of plasmid that carries the Chr R resistance gene inserted recombinant based device could beused for measurement pollutant in waste water and contaminated soil. This approach has a great potential forbioremediation of Cr (VI)-polluted environments. The bacterial isolates can be exploited for the bioremediation ofhexavalent chromium containing wastes, since it seem to have a potential to reduce the toxic hexavalent form to itsnontoxic trivalent form.
G.Renuga, M. Janeefa, “Potential of engineered Pseudomonas putida for Bioremediation of Cr (VI) tannery polluted environment”, International Journal of Advance Engineering and Research Development (IJAERD), Vol. 5, Issue 1, pp. 324-329, January 2018.








