Investigation Of Chlorpyrifos By Sensitive And Selective Spectrophotometric Method In Different Samples
Keywords:
Spectrophotometer, chlorpyrifos, p-aminoacetophenone, environmental and biological samples.Abstract
A new and highly sensitive spectrophotometric method for the determination of widely used
organophosphorus insecticide chlorpyrifosis done. The method is based on alkaline hydrolysis of chlorpyrifos to 1,2,4
trichloropyridine, followed by coupling with diazotized p-aminoacetophenone in alkaline medium. The absorption
maxima of the yellowish-orange dye formed is measured at 450 nm. Beer’s law is obeyed over the concentration
range of 4 to 35µg in a final solution volume of 25 ml (0.16 - 1.4ppm). The molar absorptivity, sandell’s sensitivity,
correlation coefficient have been determined. The method is simple sensitive and free from interferences of other
pesticides and diverse ions. The method has been satisfactorily applied to the determination of chlorpyrfos in different
environmental and biological samples.