Potential of engineered Pseudomonas putida for Bioremediation of Cr (VI) tannery polluted environment

Abstract

Bioremediation of composite tannery wastewater appears to be incompatible for direct biological treatment.
The present work aimed contaminated area can be treated with microbes that are genetically engineered for degradation
specific toxic compounds. Tannery wastewater is heavily polluted containing appreciable biodegradable matters as well
as inorganic substances like chromium, sulphide, chloride etc. Wastewater emanated from various sub-processes of
tannery operation showed variable characteristics and also differential treatability.Pseudomonas putida isolated from
tannery sludge, were examined for their tolerance to hexavalent chromium Cr (VI) and their ability to reduce Cr (VI) to
Cr(III), a detoxification process in cell suspension and cell extracts.. Pseudomonas putida could reduce Cr (VI) up to
75% from the total content of different level of dilutions could confirm in terms of their Cr (VI) reducing ability and
resistance to Cr (VI), and the Cr (VI) reduction was mainly associated with the soluble protein fraction of the cell..
P.putida used as a source of plasmid that carries the Chr R resistance gene inserted recombinant based device could be
used for measurement pollutant in waste water and contaminated soil. This approach has a great potential for
bioremediation of Cr (VI)-polluted environments. The bacterial isolates can be exploited for the bioremediation of
hexavalent chromium containing wastes, since it seem to have a potential to reduce the toxic hexavalent form to its
nontoxic trivalent form.